Emerin anticorps (AA 56-167) (CF®647)

N° du produit ABIN7653444

Cet anticorps anti-Emerin est un anticorps Souris Monoclonal détectant Emerin dans WB, IHC, IF et IHC (fp). Adapté pour Humain.

Aperçu rapide pour Emerin anticorps (AA 56-167) (CF®647) (ABIN7653444)

Antigène: Emerin (EMD)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Emerin est conjugé à/à la CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: EMD-2167

Détail du produit anti-Emerin anticorps (CF®647)

Épitope: AA 56-167

Fonction: Emerin (Papillary Thyroid Carcinoma and EDMD Marker) (EMD/2167), CF647 conjugate

Attributs du produit: Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human Emerin protein fragment (around aa 56-167)

Isotype: IgG1

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: K-562, HeLa or Jurkat cells. Kidney or Skin

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Emerin

Antigène: Emerin (EMD)

Autre désignation: Emerin

Sujet:

Synonyms: EMD, Emerin, Emery Dreifuss muscular dystrophy (EDMD), STA

Gene Symbol: EMD

Poids moléculaire: 37 kDa

ID gène: 2010

UniProt: P50402